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Issue 15, 2012
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Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

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Abstract

This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.

Graphical abstract: Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

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Publication details

The article was received on 24 Feb 2012, accepted on 16 Apr 2012 and first published on 17 Apr 2012


Article type: Paper
DOI: 10.1039/C2LC40204A
Citation: Lab Chip, 2012,12, 2743-2748
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    Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors

    D. Ogończyk, P. Jankowski and P. Garstecki, Lab Chip, 2012, 12, 2743
    DOI: 10.1039/C2LC40204A

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