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Issue 13, 2012
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On-chip continuous monitoring of motile microorganisms on an ePetri platform

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Abstract

Self-imaging Petri dish platforms with microscopy resolution, which we term ‘ePetri’, can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.

Graphical abstract: On-chip continuous monitoring of motile microorganisms on an ePetri platform

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Publication details

The article was received on 24 Jan 2012, accepted on 13 Mar 2012 and first published on 20 Mar 2012


Article type: Paper
DOI: 10.1039/C2LC40090A
Citation: Lab Chip, 2012,12, 2385-2390
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    On-chip continuous monitoring of motile microorganisms on an ePetri platform

    S. A. Lee, G. Zheng, N. Mukherjee and C. Yang, Lab Chip, 2012, 12, 2385
    DOI: 10.1039/C2LC40090A

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