Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 10, 2012
Previous Article Next Article

A programmable microfluidic cell array for combinatorial drug screening

Author affiliations

Abstract

We describe the development of a fully automatic and programmable microfluidic cell culture array that integrates on-chip generation of drug concentrations and pair-wise combinations with parallel culture of cells for drug candidate screening applications. The device has 64 individually addressable cell culture chambers in which cells can be cultured and exposed either sequentially or simultaneously to 64 pair-wise concentration combinations of two drugs. For sequential exposure, a simple microfluidic diffusive mixer is used to generate different concentrations of drugs from two inputs. For generation of 64 pair-wise combinations from two drug inputs, a novel time dependent variable concentration scheme is used in conjunction with the simple diffusive mixer to generate the desired combinations without the need for complex multi-layer structures or continuous medium perfusion. The generation of drug combinations and exposure to specific cell culture chambers are controlled using a LabVIEW interface capable of automatically running a multi-day drug screening experiment. Our cell array does not require continuous perfusion for keeping cells exposed to concentration gradients, minimizing the amount of drug used per experiment, and cells cultured in the chamber are not exposed to significant shear stress continuously. The utility of this platform is demonstrated for inducing loss of viability of PC3 prostate cancer cells using combinations of either doxorubicin or mitoxantrone with TRAIL (TNF-alpha Related Apoptosis Inducing Ligand) either in a sequential or simultaneous format. Our results demonstrate that the device can capture the synergy between different sensitizer drugs and TRAIL and demonstrate the potential of the microfluidic cell array for screening and optimizing combinatorial drug treatments for cancer therapy.

Graphical abstract: A programmable microfluidic cell array for combinatorial drug screening

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 04 Dec 2011, accepted on 20 Feb 2012 and first published on 22 Feb 2012


Article type: Paper
DOI: 10.1039/C2LC21202A
Citation: Lab Chip, 2012,12, 1813-1822
  •   Request permissions

    A programmable microfluidic cell array for combinatorial drug screening

    J. Kim, D. Taylor, N. Agrawal, H. Wang, H. Kim, A. Han, K. Rege and A. Jayaraman, Lab Chip, 2012, 12, 1813
    DOI: 10.1039/C2LC21202A

Search articles by author