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Issue 3, 2012
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Rapid, multiplexed microfluidic phage display

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Abstract

The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets. Surface display techniques allow for efficient handling of large molecular libraries in small volumes. In particular, phage display has emerged as a powerful technology for selecting peptides and proteins with enhanced, target-specific binding affinities. Yet, the process becomes cumbersome and time-consuming when multiple targets are involved. Here we demonstrate for the first time a microfluidic chip capable of identifying high affinity phage-displayed peptides for multiple targets in just a single round and without the need for bacterial infection. The chip is shown to be able to yield well-established control consensus sequences while simultaneously identifying new sequences for clinically important targets. Indeed, the confined parameters of the device allow not only for highly controlled assay conditions but also introduce a significant time-reduction to the phage display process. We anticipate that this easily-fabricated, disposable device has the potential to impact areas ranging from fundamental studies of protein, peptide, and molecular interactions, to applications such as fully automated proteomic screening.

Graphical abstract: Rapid, multiplexed microfluidic phage display

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Publication details

The article was received on 18 Nov 2011, accepted on 09 Dec 2011 and first published on 09 Dec 2011


Article type: Communication
DOI: 10.1039/C2LC21129G
Citation: Lab Chip, 2012,12, 562-565
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    Rapid, multiplexed microfluidic phage display

    K. Cung, R. L. Slater, Y. Cui, S. E. Jones, H. Ahmad, R. R. Naik and M. C. McAlpine, Lab Chip, 2012, 12, 562
    DOI: 10.1039/C2LC21129G

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