Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2012
Previous Article Next Article

A multifunctional pipette

Author affiliations

Abstract

Microfluidics has emerged as a powerful laboratory toolbox for biologists, allowing manipulation and analysis of processes at a cellular and sub-cellular level, through utilization of microfabricated features at size-scales relevant to that of a single cell. In the majority of microfluidic devices, sample processing and analysis occur within closed microchannels, imposing restrictions on sample preparation and use. We present an optimized non-contact open-volume microfluidic tool to overcome these and other restrictions, through the use of a hydrodynamically confined microflow pipette, serving as a multifunctional solution handling and dispensing tool. The geometries of the tool have been optimised for use in optical microscopy, with integrated solution reservoirs to reduce reagent use, contamination risks and cleaning requirements. Device performance was characterised using both epifluorescence and total internal reflection fluorescence (TIRF) microscopy, resulting in ∼200 ms and ∼130 ms exchange times at ∼100 nm and ∼30 μm distances to the surface respectively.

Graphical abstract: A multifunctional pipette

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 19 Sep 2011, accepted on 08 Dec 2011 and first published on 17 Jan 2012


Article type: Paper
DOI: 10.1039/C2LC20906C
Citation: Lab Chip, 2012,12, 1255-1261
  • Open access:
  •   Request permissions

    A multifunctional pipette

    A. Ainla, G. D. M. Jeffries, R. Brune, O. Orwar and A. Jesorka, Lab Chip, 2012, 12, 1255
    DOI: 10.1039/C2LC20906C

Search articles by author