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Issue 3, 2012
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Multi-gradient hydrogels produced layer by layer with capillary flow and crosslinking in open microchannels

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Abstract

This technical note describes a new bench-top method for producing anisotropic hydrogels composed of gradient layers of soluble factors, particles, polymer concentrations or material properties. Each gradient layer was produced by a previous gradient method in which a droplet of one precursor solution was added to a thin layer of a second solution. The ensuing rapid capillary flow along the open channel generated a gradient precursor solution, which was then crosslinked to form a gradient gel. Repeating these steps allowed a layered gel to be iteratively constructed with as many gradient layers as desired. This technique renders the synthesis of multi-layered gradient gels accessible to virtually any researcher and should help simplify the production of more biologically relevant cellular microenvironments.

Graphical abstract: Multi-gradient hydrogels produced layer by layer with capillary flow and crosslinking in open microchannels

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Publication details

The article was received on 13 Jun 2011, accepted on 22 Nov 2011 and first published on 14 Dec 2011


Article type: Technical Note
DOI: 10.1039/C2LC20515G
Citation: Lab Chip, 2012,12, 659-661
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    Multi-gradient hydrogels produced layer by layer with capillary flow and crosslinking in open microchannels

    F. Piraino, G. Camci-Unal, M. J. Hancock, M. Rasponi and A. Khademhosseini, Lab Chip, 2012, 12, 659
    DOI: 10.1039/C2LC20515G

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