Jump to main content
Jump to site search

Issue 4, 2012
Previous Article Next Article

Fully automated cellular-resolution vertebrate screening platform with parallel animal processing

Author affiliations

Abstract

The zebrafish larva is an optically-transparent vertebrate model with complex organs that is widely used to study genetics, developmental biology, and to model various human diseases. In this article, we present a set of novel technologies that significantly increase the throughput and capabilities of our previously described vertebrate automated screening technology (VAST). We developed a robust multi-thread system that can simultaneously process multiple animals. System throughput is limited only by the image acquisition speed rather than by the fluidic or mechanical processes. We developed image recognition algorithms that fully automate manipulation of animals, including orienting and positioning regions of interest within the microscope's field of view. We also identified the optimal capillary materials for high-resolution, distortion-free, low-background imaging of zebrafish larvae.

Graphical abstract: Fully automated cellular-resolution vertebrate screening platform with parallel animal processing

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2011, accepted on 09 Nov 2011 and first published on 08 Dec 2011


Article type: Paper
DOI: 10.1039/C1LC20849G
Citation: Lab Chip, 2012,12, 711-716
  •   Request permissions

    Fully automated cellular-resolution vertebrate screening platform with parallel animal processing

    T. Chang, C. Pardo-Martin, A. Allalou, C. Wählby and M. F. Yanik, Lab Chip, 2012, 12, 711
    DOI: 10.1039/C1LC20849G

Search articles by author

Spotlight

Advertisements