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Issue 1, 2012
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Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer

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Abstract

We describe a microfluidic cytometer that performs simultaneous optical and electrical characterisation of particles. The microfluidic chip measures side scattered light, signal extinction and fluorescence using integrated optical fibres coupled to photomultiplier tubes. The channel is 80 μm high and 200 μm wide, and made from SU-8 patterned and sandwiched between glass substrates. Particles were focused into the analysis region using 1-D hydrodynamic focusing and typical particle velocities were 0.1 ms−1. Excitation light is coupled into the detection channel with an optical fibre and focused into the channel using an integrated compound air lens. The electrical impedance of particles is measured at 1 MHz using micro-electrodes fabricated on the channel top and bottom. This data is used to accurately size the particles. The system is characterised using a range of different sized polystyrene beads (fluorescent and non-fluorescent). Single and mixed populations of beads were measured and the data compared with a conventional flow cytometer.

Graphical abstract: Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer

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Publication details

The article was received on 19 Aug 2011, accepted on 20 Oct 2011 and first published on 03 Nov 2011


Article type: Paper
DOI: 10.1039/C1LC20785G
Citation: Lab Chip, 2012,12, 118-126
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    Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer

    D. Barat, D. Spencer, G. Benazzi, M. C. Mowlem and H. Morgan, Lab Chip, 2012, 12, 118
    DOI: 10.1039/C1LC20785G

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