Jump to main content
Jump to site search

Issue 45, 2012
Previous Article Next Article

Ligand-free strategy for ultrafast and highly selective enrichment of glycopeptides using Ag-coated magnetic nanoarchitectures

Author affiliations

Abstract

Selective enrichment of glycopeptides from complicated biological samples is essential for MS-based glycoproteomics, but still remains a great challenge. In this study, we report an unprecedented ligand-free strategy for the selective enrichment of glycopeptides by simply utilizing the multivalent interaction between glycopeptides and silver nanoparticles (Ag-NPs) coated magnetic nanoarchitectures. The composite microspheres were deliberately designed to be constructed with a high-magnetic-response magnetic colloid nanocrystal cluster (MCNC) core, a poly(methacrylic acid) (PMAA) interim layer and a Ag-NPs functional shell with high coverage. Taking advantage of the reversible interaction of glycans with Ag-NPs and the high magnetic susceptibility of the magnetite core, the MCNC@PMAA@Ag-NPs microspheres possess remarkable selectivity for glycopeptides even at a low molar ratio of glycopeptides/non-glycopeptides (1 : 100) with a very rapid enrichment speed (only 1 min needed) and a simple operation procedure using magnetic separation. Applying this approach, we identified 127 unique glycopeptides mapped to 51 different glycoproteins from only 1 μL rat serum samples. These results clearly demonstrated that the MCNC@PMAA@Ag-NPs have great potential for purifying and identifying the low-abundant glycopeptides in complex biological samples.

Graphical abstract: Ligand-free strategy for ultrafast and highly selective enrichment of glycopeptides using Ag-coated magnetic nanoarchitectures

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Aug 2012, accepted on 01 Oct 2012 and first published on 01 Oct 2012


Article type: Paper
DOI: 10.1039/C2JM35196J
Citation: J. Mater. Chem., 2012,22, 23981-23988
  •   Request permissions

    Ligand-free strategy for ultrafast and highly selective enrichment of glycopeptides using Ag-coated magnetic nanoarchitectures

    W. Ma, L. Li, Y. Zhang, Q. An, L. You, J. Li, Y. Zhang, S. Xu, M. Yu, J. Guo, H. Lu and C. Wang, J. Mater. Chem., 2012, 22, 23981
    DOI: 10.1039/C2JM35196J

Search articles by author

Spotlight

Advertisements