Jump to main content
Jump to site search

Issue 37, 2012
Previous Article Next Article

Biomimetic supported lipid bilayers with high cholesterol content formed by α-helical peptide-induced vesicle fusion

Author affiliations

Abstract

In this study, we present a technique to create complex, high cholesterol-containing supported lipid bilayers (SLBs) using α-helical (AH) peptide-induced vesicle fusion. Vesicles consisting of POPC : POPE : POPS : SM : Chol (9.35 : 19.25 : 8.25 : 18.15 : 45.00) were used to form a SLB that models the native composition of the human immunodeficiency virus-1 (HIV-1) lipid envelope. In the absence of AH peptides, these biomimetic vesicles fail to form a complete SLB. We verified and characterized AH peptide-induced vesicle fusion by a quartz crystal microbalance with dissipation monitoring, neutron reflectivity, and atomic force microscopy. Successful SLB formation entailed a characteristic frequency shift of −35.4 ± 0.8 Hz and a change in dissipation energy of 1.91 ± 0.23 × 10−6. Neutron reflectivity measurements determined the SLB thickness to be 49.9 +1.9−1.5 Å, and showed the SLB to be 100 +0.0−0.1% complete and void of residual AH peptide after washing. Atomic force microscopy imaging confirmed complete SLB formation and revealed three distinct domains with no visible defects. This vesicle fusion technique gives researchers access to a complex SLB composition with high cholesterol content and thus the ability to better recapitulate the native HIV-1 lipid membrane.

Graphical abstract: Biomimetic supported lipid bilayers with high cholesterol content formed by α-helical peptide-induced vesicle fusion

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 Mar 2012, accepted on 24 May 2012 and first published on 27 Jun 2012


Article type: Paper
DOI: 10.1039/C2JM32016A
Citation: J. Mater. Chem., 2012,22, 19506-19513
  •   Request permissions

    Biomimetic supported lipid bilayers with high cholesterol content formed by α-helical peptide-induced vesicle fusion

    G. J. Hardy, R. Nayak, S. Munir Alam, J. G. Shapter, F. Heinrich and S. Zauscher, J. Mater. Chem., 2012, 22, 19506
    DOI: 10.1039/C2JM32016A

Search articles by author

Spotlight

Advertisements