Jump to main content
Jump to site search

Issue 8, 2012
Previous Article Next Article

Functional characterization and axonal transport of quantum dot labeled BDNF

Author affiliations

Abstract

Brain derived neurotrophic factor (BDNF) plays a key role in the growth, development and maintenance of the central and peripheral nervous systems. Exogenous BDNF activates its membrane receptors at the axon terminal, and subsequently sends regulation signals to the cell body. To understand how a BDNF signal propagates in neurons, it is important to follow the trafficking of BDNF after it is internalized at the axon terminal. Here we labeled BDNF with bright, photostable quantum dots (QD-BDNF) and followed the axonal transport of QD-BDNF in real time in hippocampal neurons. We showed that QD-BDNF was able to bind BDNF receptors and activate downstream signaling pathways. When QD-BDNF was applied to the distal axons of hippocampal neurons, it was observed to be actively transported toward the cell body at an average speed of 1.11 ± 0.05 μm s−1. A closer examination revealed that QD-BDNF was transported by both discrete endosomes and multivesicular body-like structures. Our results showed that QD-BDNF could be used to track the movement of exogenous BDNF in neurons over long distances and to study the signaling organelles that contain BDNF.

Graphical abstract: Functional characterization and axonal transport of quantum dot labeled BDNF

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Mar 2012, accepted on 12 Jun 2012 and first published on 14 Jun 2012


Article type: Technical Innovation
DOI: 10.1039/C2IB20062G
Citation: Integr. Biol., 2012,4, 953-960
  •   Request permissions

    Functional characterization and axonal transport of quantum dot labeled BDNF

    W. Xie, K. Zhang and B. Cui, Integr. Biol., 2012, 4, 953
    DOI: 10.1039/C2IB20062G

Search articles by author

Spotlight

Advertisements