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Issue 8, 2012
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Changes in thyroid peroxidase activity in response to various chemicals

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Abstract

Thyroperoxidase (TPO) is a large heme-containing glycoprotein that catalyzes the transfer of iodine to thyroglobulin during thyroid hormone (TH) synthesis. Previously, we established an in vitro assay for TPO activity based on human recombinant TPO (hrTPO) stably transfected into human follicular thyroid carcinoma (FTC-238) cells. It is important to determine whether environmental chemicals can disrupt TPO activity because it is an important factor in the TH axis. In this study, we used our assay to examine the changes in TPO activity in response to various chemicals, including benzophenones (BPs), polycyclic aromatic hydrocarbons (PAHs), and persistent organic pollutants (POPs). Overall, BPs, PAHs, and POPs slightly altered TPO activity at low doses, as compared with the positive controls methimazole (MMI), genistein, and 2,2′,4,4′-tetrahydroxy BP. Benzophenone, benzhydrol, 3-methylchloranthracene, pyrene, benzo(k)fluoranthene, benzo(e)pyrene, perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and heptachlor decreased TPO activity, while 2,4-dihydroxy BP, 2,2′-dihydroxy-4-methoxy BP, and dibenzo(a,h)anthracene increased TPO activity. From these data, we can predict the disruption of TPO activity by various chemicals as a sensitive TH end point. TPO activity should be considered when enacting measures to regulate environmental exposure to thyroid-disrupting chemicals.

Graphical abstract: Changes in thyroid peroxidase activity in response to various chemicals

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Publication details

The article was received on 06 Feb 2012, accepted on 19 Apr 2012 and first published on 01 May 2012


Article type: Paper
DOI: 10.1039/C2EM30106G
Citation: J. Environ. Monit., 2012,14, 2121-2126
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    Changes in thyroid peroxidase activity in response to various chemicals

    M. Song, Y. Kim, Y. Park and J. Ryu, J. Environ. Monit., 2012, 14, 2121
    DOI: 10.1039/C2EM30106G

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