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Issue 1, 2012
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Statistical evaluation of photon count rate data for nanoscale particle measurement in wastewaters

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Abstract

The dynamic light scattering (DLS) technique can detect the concentration and size distribution of nanoscale particles in aqueous solutions by analyzing photon interactions. This study evaluated the applicability of using photon count rate data from DLS analyses for measuring levels of biogenic and manufactured nanoscale particles in wastewater. Statistical evaluations were performed using secondary wastewater effluent and a Malvern Zetasizer. Dynamic light scattering analyses were performed equally by two analysts over a period of two days using five dilutions and twelve replicates for each dilution. Linearity evaluation using the sixty sample analysis yielded a regression coefficient R2 = 0.959. The accuracy analysis for various dilutions indicated a recovery of 100 ± 6%. Precision analyses indicated low variance coefficients for the impact of analysts, days, and within sample error. The variation by analysts was apparent only in the most diluted sample (intermediate precision ∼12%), where the photon count rate was close to the instrument detection limit. The variation for different days was apparent in the two most concentrated samples, which indicated that wastewater samples must be analyzed for nanoscale particle measurement within the same day of collection. Upon addition of 10 mg l−1 of nanosilica to wastewater effluent samples, the measured photon count rates were within 5% of the estimated values. The results indicated that photon count rate data can effectively complement various techniques currently available to detect nanoscale particles in wastewaters.

Graphical abstract: Statistical evaluation of photon count rate data for nanoscale particle measurement in wastewaters

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Publication details

The article was received on 15 Mar 2011, accepted on 06 Oct 2011 and first published on 03 Nov 2011


Article type: Paper
DOI: 10.1039/C1EM10237K
Citation: J. Environ. Monit., 2012,14, 79-84
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    Statistical evaluation of photon count rate data for nanoscale particle measurement in wastewaters

    J. Smeraldi, R. Ganesh, J. Safarik and D. Rosso, J. Environ. Monit., 2012, 14, 79
    DOI: 10.1039/C1EM10237K

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