Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2012
Previous Article Next Article

The structure and activation of substrate water molecules in the S2 state of photosystem II studied by hyperfine sublevel correlation spectroscopy

Author affiliations

Abstract

The water-splitting protein, photosystem II, catalyzes the light-driven oxidation of water to dioxygen. The solar water oxidation reaction takes place at the catalytic center, referred to as the oxygen-evolving complex, of photosystem II. During the catalytic cycle, the oxygen-evolving complex cycles through five distinct intermediate states, S0–S4. In this study, we trap the oxygen-evolving complex in the S2 intermediate state by low temperature illumination of photosystem II isolated from three different species, Thermosynechococcus vulcanus, the PsbB variant of Synechocystis PCC 6803 and spinach. We apply two-dimensional hyperfine sublevel correlation spectroscopy to detect weak magnetic interactions between the paramagnetic tetra-nuclear manganese cluster of the S2 state of the OEC and the surrounding protons. We identify five groups of protons that are interacting with the tetra-nuclear manganese cluster. From the values of hyperfine interactions and using the recently reported 1.9 Å resolution X-ray structure of the OEC in the S1 state [Umena et al., Nature, 2011, 473, 55], we discuss the assignments of the five groups of protons and draw important conclusions on the structure of the oxygen-evolving complex in the S2 state. In addition, we conclude that the structure of OEC is nearly identical in photosystem II from Thermosynechococcus vulcanus, the PsbB variant of Synechocystis PCC 6803 and spinach.

Graphical abstract: The structure and activation of substrate water molecules in the S2 state of photosystem II studied by hyperfine sublevel correlation spectroscopy

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 25 Jan 2012, accepted on 19 Apr 2012 and first published on 19 Apr 2012


Article type: Paper
DOI: 10.1039/C2EE21210B
Citation: Energy Environ. Sci., 2012,5, 7747-7756
  •   Request permissions

    The structure and activation of substrate water molecules in the S2 state of photosystem II studied by hyperfine sublevel correlation spectroscopy

    S. Milikisiyants, R. Chatterjee, C. S. Coates, F. H. M. Koua, J. Shen and K. V. Lakshmi, Energy Environ. Sci., 2012, 5, 7747
    DOI: 10.1039/C2EE21210B

Search articles by author