Jump to main content
Jump to site search

Issue 15, 2012
Previous Article Next Article

DNA binding and antiproliferative activity toward human carcinoma cells of copper(II) and zinc(II) complexes of a 2,5-diphenyl[1,3,4]oxadiazole derivative

Author affiliations

Abstract

The interaction of calf thymus DNA with [CuL(ClO4)]ClO4·H2O (1) and [ZnLBr]Br·H2O (2) (L = 9,12,15,18,27,28-hexaaza-29-oxatetracyclo[24.2.1.02,7.020,25]enneicosa-2,4,6,20,22,24,26,281-octaene) dicationic complexes in aqueous solution at neutral pH, was investigated by variable-temperature UV-vis absorption, circular dichroism and fluorescence spectroscopy. The values of the DNA-binding constants of these complexes, determined by competitive binding spectrofluorimetric titrations of ethidium bromide (EB)–DNA solutions, are (6.7 ± 0.5) × 106 M−1 for CuL2+ and (4.7 ± 0.5) × 105 M−1 for ZnL2+. These data together with a through analysis of the spectroscopic behaviour consistently suggest that both compounds are effective DNA binders. Interestingly, the DNA-binding strength of these complexes has been found to be correlated to their in vitro cytotoxic activity toward human breast carcinoma cells, although the complex with lower DNA-binding affinity is more active. In fact, biological studies showed that when the compounds are delivered through the cell membrane by a lipidic carrier, the cell survival is sensibly reduced, up to 58% with 1 and to 31% with 2.

Graphical abstract: DNA binding and antiproliferative activity toward human carcinoma cells of copper(ii) and zinc(ii) complexes of a 2,5-diphenyl[1,3,4]oxadiazole derivative

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 15 Sep 2011, accepted on 09 Jan 2012 and first published on 15 Feb 2012


Article type: Paper
DOI: 10.1039/C2DT11759B
Citation: Dalton Trans., 2012,41, 4389-4395
  •   Request permissions

    DNA binding and antiproliferative activity toward human carcinoma cells of copper(II) and zinc(II) complexes of a 2,5-diphenyl[1,3,4]oxadiazole derivative

    A. Terenzi, M. Fanelli, G. Ambrosi, S. Amatori, V. Fusi, L. Giorgi, V. Turco Liveri and G. Barone, Dalton Trans., 2012, 41, 4389
    DOI: 10.1039/C2DT11759B

Search articles by author