Jump to main content
Jump to site search

Issue 2, 2013
Previous Article Next Article

The unusual stabilization of the Ni2+ and Cu2+ complexes with NSFRY

Author affiliations

Abstract

The binding mode provided by an unprotected peptide with non-coordinating side-chains is simple and well understood. However, when particular residues are inserted into the peptide sequence, they can have a significant impact on the stability of the formed complexes. The presence of non-bonding side chains of amino acids close to the metal binding centre in the peptide/protein can provide special interactions which result in increasing the stabilization of the formed species. Moreover, these interactions can play a crucial role in generating particular protein structures and in influencing biological activity. In the present paper it is shown how peptides with no specific predisposition for metal binding, like ANF peptides, can form metal complexes with a very high thermodynamic stability. For better understanding this peculiar behavior, a combined pH-metric and spectroscopic method was used to determine the stability and the solution structure of Cu2+ and Ni2+ complexes with NSFRY-NH2 (ANF peptide) and a series of analogue peptides. All obtained data support the hypothesis that the complex-formation process is very similar for both metal ions and all the ligands, involving some intramolecular interactions among the different side chains. The two-dimensional NMR analysis of nickel complexes showed the occurrence of many inter-residue correlations and suggested the presence of a direct interaction between the d electrons of the metal ion and the π-ring system of the aromatic side-chains of the ligand.

Graphical abstract: The unusual stabilization of the Ni2+ and Cu2+ complexes with NSFRY

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 27 Aug 2012, accepted on 05 Oct 2012 and first published on 09 Oct 2012


Article type: Paper
DOI: 10.1039/C2DT31959D
Citation: Dalton Trans., 2013,42, 448-458
  •   Request permissions

    The unusual stabilization of the Ni2+ and Cu2+ complexes with NSFRY

    A. Janicka-Klos, E. Porciatti, D. Valensin, C. Conato, M. Remelli, S. Oldziej, G. Valensin and H. Kozlowski, Dalton Trans., 2013, 42, 448
    DOI: 10.1039/C2DT31959D

Search articles by author