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Issue 6, 2012
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Studies on characterization, telomerase inhibitory properties and G-quadruplex binding of η6-arene ruthenium complexes with 1,10-phenanthroline-derived ligands

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Abstract

Two arene ruthenium complexes [Ru(η6-C6H6)(p-MOPIP)Cl]+1 and [Ru(η6-C6H6)(p-CFPIP)Cl]+2, where p-MOPIP = 2-(4-methoxyphenyl)-imidazo[4,5f][1,10] phenanthroline and p-CFPIP = 2-(4-trifluoromethylphenyl)-imidazo[4,5f][1,10] phenanthroline, were prepared and the interactions of these compounds with DNA oligomers 5′-G3(T2AG3)3-3′(HTG21) have been studied by UV-vis and circular dichroism (CD) spectroscopy, gel mobility shift assay, fluorescence resonance energy transfer (FRET) melting assay, polymerase chain reaction (PCR) stop assay and telomeric repeat amplification protocol (TRAP) assay. The results show that both complexes can induce the stabilization of quadruplex DNA but complex 1 is a better G-quadruplex binder than complex 2. The two ruthenium complexes tested led to an inhibition of the enzyme telomerase and complex 1 was the significantly better inhibitor. A novel visual method has been developed for making a distinction between G-quadruplex DNA and double DNA by our Ru complexes binding hemin to form the hemin-G-quadruplex DNAzyme. Furthermore, in vitro cytotoxicity studies showed complex 1 exhibited quite potent antitumor activities and the greatest inhibitory selectivity against cancer cell lines.

Graphical abstract: Studies on characterization, telomerase inhibitory properties and G-quadruplex binding of η6-arene ruthenium complexes with 1,10-phenanthroline-derived ligands

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Publication details

The article was received on 05 Sep 2011, accepted on 26 Oct 2011 and first published on 08 Dec 2011


Article type: Paper
DOI: 10.1039/C1DT11676B
Citation: Dalton Trans., 2012,41, 1734-1741
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    Studies on characterization, telomerase inhibitory properties and G-quadruplex binding of η6-arene ruthenium complexes with 1,10-phenanthroline-derived ligands

    D. Sun, R. Zhang, F. Yuan, D. Liu, Y. Zhou and J. Liu, Dalton Trans., 2012, 41, 1734
    DOI: 10.1039/C1DT11676B

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