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Issue 4, 2012
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Construction of biocatalysts using the myoglobin scaffold for the synthesis of indigo from indole

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Abstract

Hydrogen peroxide-dependent oxidation of indole producing indigo has been catalyzed by engineered myoglobins (Mbs). We have investigated a series of H64D mutants of Mbs in which Asp-64 accelerates the reaction of the Mbs with hydrogen peroxide and stabilizes the resulting active species: compound I (iron oxo-ferryl porphyrin π-cation radical). The catalytic activity of mutants depends on the amino acids at 68 and 107 positions and the H64D/V68I/I107A mutant gave the highest catalytic activity, suggesting that the side chains of Ile-68 and Ala-107 provide a hydrophobic environment suitable for indole binding. The kcat of the H64D/V68I/I107A mutant achieved 72 min−1, indicating that even Mb can be transformed into a biocatalyst.

Graphical abstract: Construction of biocatalysts using the myoglobin scaffold for the synthesis of indigo from indole

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Publication details

The article was received on 17 Oct 2011, accepted on 24 Nov 2011 and first published on 13 Dec 2011


Article type: Paper
DOI: 10.1039/C2CY00427E
Citation: Catal. Sci. Technol., 2012,2, 739-744
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    Construction of biocatalysts using the myoglobin scaffold for the synthesis of indigo from indole

    J. Xu, O. Shoji, T. Fujishiro, T. Ohki, T. Ueno and Y. Watanabe, Catal. Sci. Technol., 2012, 2, 739
    DOI: 10.1039/C2CY00427E

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