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Issue 32, 2012
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Binding motifs of silver in prion octarepeat model peptides: a joint ion mobility, IR and UV spectroscopies, and theoretical approach

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Abstract

Transition metal-ion complexation is essential to the function and structural stability of many proteins. We studied silver complexation with the octarepeat motif ProHisGlyGlyGlyTrpGlyGln of the prion protein, which shows competitive sites for metal chelation including amide, indole and imidazole groups. This octapeptide is known as a favourable transition metal binding site in prion protein. We used ion mobility spectrometry (IMS), infrared multiple photon dissociation (IRMPD) spectroscopy and density functional theory calculations (DFT) to identify the binding motifs of a silver cation on HisGlyGlyGlyTrp peptide as well as on peptide subsequences. Ultra-violet photodissociation (UVPD) and collision induced dissociation mass spectrometry together with the time-dependent density functional method was then exploited to study the influence of binding sites on optical properties and on the ground and excited states reactivity of the peptide. We show that the metal cation is bound to the π-system of the indole group and a nitrogen atom of the imidazole group and that charge transfers from the indole group to the silver cation occur in excited electronic states.

Graphical abstract: Binding motifs of silver in prion octarepeat model peptides: a joint ion mobility, IR and UV spectroscopies, and theoretical approach

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Publication details

The article was received on 23 Mar 2012, accepted on 31 May 2012 and first published on 01 Jun 2012


Article type: Paper
DOI: 10.1039/C2CP40924K
Citation: Phys. Chem. Chem. Phys., 2012,14, 11433-11440
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    Binding motifs of silver in prion octarepeat model peptides: a joint ion mobility, IR and UV spectroscopies, and theoretical approach

    B. Bellina, I. Compagnon, L. MacAleese, F. Chirot, J. Lemoine, P. Maître, M. Broyer, R. Antoine, A. Kulesza, R. Mitrić, V. Bonačić-Koutecký and P. Dugourd, Phys. Chem. Chem. Phys., 2012, 14, 11433
    DOI: 10.1039/C2CP40924K

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