Folding super-sized DNA origami with scaffold strands from long-range PCR

Honglu Zhang; Jie Chao; Dun Pan; Huajie Liu; Qing Huang; Chunhai Fan

doi:10.1039/C2CC32204H

Folding super-sized DNA origami with scaffold strands from long-range PCR

Honglu Zhang,^a Jie Chao,*^a Dun Pan,^a Huajie Liu,^a Qing Huang^a and Chunhai Fan*^a

* Corresponding authors

^a Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
E-mail: chaojie@sinap.ac.cn, fchh@sinap.ac.cn

Abstract

A 26 kilobase single strand DNA fragment was obtained from long-range PCR amplification and subsequent enzymatic digestion, which we folded into a super-sized DNA origami nanostructure by using ∼800 staple strands.

Supplementary files

Publication details

The article was received on 27 Mar 2012, accepted on 23 Apr 2012 and first published on 24 Apr 2012

Article type: Communication

DOI: 10.1039/C2CC32204H

Citation: Chem. Commun., 2012,48, 6405-6407

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Folding super-sized DNA origami with scaffold strands from long-range PCR

H. Zhang, J. Chao, D. Pan, H. Liu, Q. Huang and C. Fan, Chem. Commun., 2012, 48, 6405
DOI: 10.1039/C2CC32204H
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Chemical Communications

Folding super-sized DNA origami with scaffold strands from long-range PCR

Abstract

Supplementary files

Publication details

Folding super-sized DNA origami with scaffold strands from long-range PCR

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