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Issue 18, 2012
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A new stable and reliable method for labeling nucleic acids of fully replicative viruses

Li-Li Huang,a  Peng Zhou,b  Han-Zhong Wang,c  Rui Zhang,a  Jian Hao,a  Hai-Yan Xie*a  and  Zhi-Ke He*b 
Author affiliations

* Corresponding authors

a School of Life Science, Beijing Institute of Technology, Beijing 100081, China
E-mail: hyanxie@gmail.com

b College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China
E-mail: zhkhe@whu.edu.cn

c Wuhan Institute of Virology, Chinese Academy of Sciences, China

Abstract

Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a ‘molecular light switch’ complex [Ru(phen)2(dppz)]2+, where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2′,3′-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.

Graphical abstract: A new stable and reliable method for labeling nucleic acids of fully replicative viruses

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Supplementary files

Publication details

The article was received on 14 Nov 2011, accepted on 10 Jan 2012 and first published on 10 Jan 2012


Article type: Communication
DOI: 10.1039/C2CC17069H
Citation: Chem. Commun., 2012,48, 2424-2426
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    A new stable and reliable method for labeling nucleic acids of fully replicative viruses

    L. Huang, P. Zhou, H. Wang, R. Zhang, J. Hao, H. Xie and Z. He, Chem. Commun., 2012, 48, 2424
    DOI: 10.1039/C2CC17069H

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