Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

Yadveer S. Grewal; Muhammad J. A. Shiddiky; Sean A. Gray; Kris M. Weigel; Gerard A. Cangelosi; Matt Trau

doi:10.1039/C2CC38882K

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Chemical Communications

Issue 15, 2013

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Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

Yadveer S. Grewal,^a Muhammad J. A. Shiddiky,*^a Sean A. Gray,^b Kris M. Weigel,^b Gerard A. Cangelosi^b and Matt Trau*^a

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Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia
E-mail: m.shiddiky@uq.edu.au ;
Fax: +61-7-33463973 ;
Tel: +61-7-33464178

Seattle Biomedical Research Institute, Seattle, USA

Chem. Commun., 2013,49, 1551-1553

DOI: 10.1039/C2CC38882K
Received 12 Dec 2012, Accepted 07 Jan 2013
First published online 07 Jan 2013

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Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

Abstract from Analytical Abstracts 2013

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Yadveer S. Grewal
Muhammad J. A. Shiddiky
Sean A. Gray
Kris M. Weigel
Gerard A. Cangelosi
Matt Trau

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Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

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