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Simple and general cancer cell detection methods are required in point-of-care diagnostics. Herein, the interaction between an anticancer drug, daunomycin, and cancer cell membrane components has been studied using an aptamer probe immobilized on a conducting polymer–gold nanoparticle composite film through electrochemical and fluorescence methods and applied to the quantitative detection of cancer cells. The developed method differentiates between cancerous and noncancerous cells effectively.
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