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Issue 14, 2013
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A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

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Abstract

Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O2 oxidation. 17O-NMR with the aid of 17O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Graphical abstract: A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

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Publication details

The article was received on 24 Sep 2012, accepted on 02 Jan 2013 and first published on 03 Jan 2013


Article type: Communication
DOI: 10.1039/C2CC36918D
Citation: Chem. Commun., 2013,49, 1449-1451
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    A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

    S. Hanashima, N. Fujiwara, K. Matsumoto, N. Iwasaki, G. Zheng, H. Torigoe, K. Suzuki, N. Taniguchi and Y. Yamaguchi, Chem. Commun., 2013, 49, 1449
    DOI: 10.1039/C2CC36918D

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