Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

Junya Chiba; Takahide Kouno; Shun Aoki; Hitoshi Sato; JingYing Zhang; Hiroshi Matsuo; Masahiko Inouye

doi:10.1039/C2CC36779C

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Chemical Communications

Issue 99, 2012

Urgent high quality communications from across the chemical sciences.

Impact Factor 6.169 100 Issues per Year Indexed in MEDLINE

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Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

Junya Chiba,*^a Takahide Kouno,*^b Shun Aoki,^a Hitoshi Sato,^a JingYing Zhang,^b Hiroshi Matsuo^b and Masahiko Inouye^a

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Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan
E-mail: chiba@pha.u-toyama.ac.jp

Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, USA
E-mail: konox005@umn.edu

Chem. Commun., 2012,48, 12115-12117

DOI: 10.1039/C2CC36779C
Received 18 Sep 2012, Accepted 30 Oct 2012
First published online 31 Oct 2012

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APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

Abstract from Analytical Abstracts 2013

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Junya Chiba
Takahide Kouno
Shun Aoki
Hitoshi Sato
JingYing Zhang
Hiroshi Matsuo
Masahiko Inouye

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