Issue 89, 2012
From the journal:

Chemical Communications

Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases†

Megan Wheeler,a   Antoine Chardon,a   Astrid Goubet,a   Kunihiko Morihiro,b   Sze Yee Tsan,a   Stacey L. Edwards,a   Tetsuya Kodama,bc   Satoshi Obikab  and  Rakesh N. Veedu*a  

* Corresponding authors

a School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, Australia
E-mail: rakesh@uq.edu.au
Fax: +61 7 33654699
Tel: +61 7 33654611

b Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita 565-0871, Japan

c Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

Abstract

Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.

Graphical abstract: Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases

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Article information

DOI
https://doi.org/10.1039/C2CC36464F
Article type
Communication
Submitted
05 Sep 2012
Accepted
24 Sep 2012
First published
27 Sep 2012

Chem. Commun., 2012,48, 11020-11022

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Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases

M. Wheeler, A. Chardon, A. Goubet, K. Morihiro, S. Y. Tsan, S. L. Edwards, T. Kodama, S. Obika and R. N. Veedu, Chem. Commun., 2012, 48, 11020 DOI: 10.1039/C2CC36464F

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