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Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, 600 S Mathews Ave, Urbana, USA
; Fax: +1 217 244 8533
; Tel: +1 217 244 5360
Chem. Commun., 2012,48, 10966-10968
31 Aug 2012,
19 Sep 2012
First published online
04 Oct 2012
We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.
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