Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe

Xibo Shen; Qiao Jiang; Jinye Wang; Luru Dai; Guozhang Zou; Zhen-Gang Wang; Wei-Qiang Chen; Wei Jiang; Baoquan Ding

doi:10.1039/C2CC36185J

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Chemical Communications

Issue 92, 2012

Urgent high quality communications from across the chemical sciences.

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Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe

Xibo Shen,^a Qiao Jiang,^b Jinye Wang,^b Luru Dai,^b Guozhang Zou,^b Zhen-Gang Wang,^b Wei-Qiang Chen,^c Wei Jiang*^a and Baoquan Ding*^b

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School of Chemistry and Chemical Engineering, Shandong University, P. R. China
E-mail: wjiang@sdu.edu.cn ;
Fax: +86 531 8856446 ;
Tel: +86 531 88363888

National Center for Nanoscience and Technology, 11 Bei Yi Tiao, ZhongGuanCun, P. R. China
E-mail: dingbq@nanoctr.cn ;
Fax: +86 10 62656765 ;
Tel: +86 10 82545636

Institute of Modern Physics, Chinese Academy of Sciences, P. R. China

Chem. Commun., 2012,48, 11301-11303

DOI: 10.1039/C2CC36185J
Received 25 Aug 2012, Accepted 27 Sep 2012
First published online 28 Sep 2012

This article is part of themed collection: Nucleic acids: new life, new materials

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We report a label-free fluorescent strategy to study the distribution and stability of DNA origami nanostructures in live, cellular systems, using carbazole-based biscyanine as a probe molecule which has the characteristic property of restriction of intramolecular rotation (RIR) induced emission.

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Xibo Shen
Qiao Jiang
Jinye Wang
Luru Dai
Guozhang Zou
Zhen-Gang Wang
Wei-Qiang Chen
Wei Jiang
Baoquan Ding

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