A highly selective fluorescent probe for fast detection of hydrogen sulfide in aqueous solution and living cells

Zheng Xu; Lin Xu; Ji Zhou; Yufang Xu; Weiping Zhu; Xuhong Qian

doi:10.1039/C2CC36141H

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Chemical Communications

Issue 88, 2012

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A highly selective fluorescent probe for fast detection of hydrogen sulfide in aqueous solution and living cells

Zheng Xu,^a Lin Xu,^a Ji Zhou,^a Yufang Xu,^a Weiping Zhu^a and Xuhong Qian*^a

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Shanghai Key Laboratory of Chemical Biology, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China
E-mail: xhqian@ecust.edu.cn ;
Fax: +86 21 6425 2603 ;
Tel: +86 21 6425 3589

Chem. Commun., 2012,48, 10871-10873

DOI: 10.1039/C2CC36141H
Received 16 Jul 2012, Accepted 14 Sep 2012
First published online 14 Sep 2012

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Abstract
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A new ratiometric fluorescence probe E1 based on an excited-state intramolecular proton transfer (ESIPT) mechanism for detection of hydrogen sulfide (H₂S) is reported. E1 responds to H₂S quickly and showed a 30-fold fluorescence enhancement in 2 minutes. Moreover, E1 can detect H₂S quantitatively with a detection limit as low as 0.12 μM in aqueous solution. Its potential for biological applications was confirmed by employing it for fluorescence imaging of H₂S in living cells.

Abstract from Analytical Abstracts 2012

A new ratiometric fluorescence probe E1 based on an excited-state intramolecular proton transfer (ESIPT) mechanism for detection of hydrogen sulfide (H₂S) is reported. E1 responds to H₂S quickly and showed a 30-fold fluorescence enhancement in 2 minutes. Moreover, E1 can detect H₂S quantitatively with a detection limit as low as 0.12 µM in aqueous solution. Its potential for biological applications was confirmed by employing it for fluorescence imaging of H₂S in living cells.

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Zheng Xu
Lin Xu
Ji Zhou
Yufang Xu
Weiping Zhu
Xuhong Qian

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