Effects of fullerene derivatives on bioluminescence and application for protease detection

Junping Yu; Mirong Guan; Fengyun Li; Zhiping Zhang; Chunru Wang; Chunying Shu; Hongping Wei; Xian-En Zhang

doi:10.1039/C2CC36099C

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Chemical Communications

Issue 89, 2012

Urgent high quality communications from across the chemical sciences.

Impact Factor 6.169 100 Issues per Year Indexed in MEDLINE

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Effects of fullerene derivatives on bioluminescence and application for protease detection

Junping Yu,^a Mirong Guan,^b^c Fengyun Li,^a^c Zhiping Zhang,^a Chunru Wang,^b Chunying Shu,*^b Hongping Wei*^a and Xian-En Zhang*^a

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State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, P. R. China
E-mail: hpwei@wh.iov.cn ;
Fax: +86-27-87199492 ;
Tel: +86-27-87199115

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, P. R. China
E-mail: shucy@iccas.ac.cn ;
Fax: +86-27-87199492 ;
Tel: +86-27-87199115

Gradual School, Chinese Academy of Sciences, Beijing, 10049, P. R. China

Chem. Commun., 2012,48, 11011-11013

DOI: 10.1039/C2CC36099C
Received 22 Jul 2012, Accepted 19 Sep 2012
First published online 21 Sep 2012

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Abstract
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The effects of two C₆₀ derivatives: C₆₀[C(COOH)₂]₃ and C₆₀(NH₂)_x(OH)_y, have been investigated on the bioluminescence of humanized Gaussia luciferase (hGluc) for the first time. Utilizing the dual properties of carboxyl C₆₀ with luminescence quenching and free radicals scavenging, a novel BRET system was constructed for protease detection with high sensitivity.

Abstract from Analytical Abstracts 2012

The effects of two C₆₀ derivatives: C₆₀(C(COOH)₂)₃ and C₆₀(NH₂)_X(OH)_Y, have been investigated on the bioluminescence of humanized Gaussia luciferase (hGluc) for the first time. Utilizing the dual properties of carboxyl C₆₀ with luminescence quenching and free radicals scavenging, a novel BRET system was constructed for protease detection with high sensitivity.

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Junping Yu
Mirong Guan
Fengyun Li
Zhiping Zhang
Chunru Wang
Chunying Shu
Hongping Wei
Xian-En Zhang

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