Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Montserrat Shelbourne; Tom Brown; Afaf H. El-Sagheer; Tom Brown

doi:10.1039/C2CC35084J

li.more_link:hover .more_menu_body_div { visibility: visible !important; display: block !important; background-color: #272521; } li.more_link:hover .masthead-menu-body { visibility: visible !important; display: block !important; } li.account_container:hover div.account_popup { visibility: visible !important; display: block !important; } li.li_cart:hover div.cart_popup { visibility: visible !important; display: block !important; }

Home > Journals > Chemical Communications > Fast and efficient DNA ...

For Authors & Referees | For Librarians | For Members

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Chemical Communications

Issue 91, 2012

Urgent high quality communications from across the chemical sciences.

Impact Factor 6.718 100 Issues per Year Indexed in MEDLINE

Journal Home

Previous Article | Next Article

Communication

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Montserrat Shelbourne,^a Tom Brown, Jr.,^b Afaf H. El-Sagheer*^a^c and Tom Brown*^a

Show Affiliations

Hide Affiliations

Corresponding authors

School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK
E-mail: tb2@soton.ac.uk, A.H.El-Sagheer@soton.ac.uk;
Fax: +44 (0)2380 592991 ;
Tel: +44 (0)2380 592974

ATDBio Ltd, Highfield, Southampton SO17 1BJ, UK

Chemistry Branch, Dept. of Science and Mathematics, Suez Canal University, Suez 43721, Egypt

Chem. Commun., 2012,48, 11184-11186

DOI: 10.1039/C2CC35084J
Received 16 Jul 2012, Accepted 01 Aug 2012
First published online 03 Aug 2012

This article is part of themed collection: Nucleic acids: new life, new materials

Please wait while Download options loads

Download Citation

Request Permissions

Abstract
Cited by
Related Content

Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.