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Issue 91, 2012
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Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

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Abstract

Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.

Graphical abstract: Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

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The article was received on 16 Jul 2012, accepted on 01 Aug 2012 and first published on 03 Aug 2012


Article type: Communication
DOI: 10.1039/C2CC35084J
Citation: Chem. Commun., 2012,48, 11184-11186
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    Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

    M. Shelbourne, T. Brown, A. H. El-Sagheer and T. Brown, Chem. Commun., 2012, 48, 11184
    DOI: 10.1039/C2CC35084J

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