Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Montserrat Shelbourne; Tom Brown; Afaf H. El-Sagheer; Tom Brown

doi:10.1039/C2CC35084J

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Montserrat Shelbourne,^a Tom Brown, Jr.,^b Afaf H. El-Sagheer*^a^c and Tom Brown*^a

* Corresponding authors

^a School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK
E-mail: tb2@soton.ac.uk, A.H.El-Sagheer@soton.ac.uk
Fax: +44 (0)2380 592991
Tel: +44 (0)2380 592974

^b ATDBio Ltd, Highfield, Southampton SO17 1BJ, UK

^c Chemistry Branch, Dept. of Science and Mathematics, Suez Canal University, Suez 43721, Egypt

Abstract

Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.

Supplementary files

Publication details

The article was received on 16 Jul 2012, accepted on 01 Aug 2012 and first published on 03 Aug 2012

Article type: Communication

DOI: 10.1039/C2CC35084J

Citation: Chem. Commun., 2012,48, 11184-11186

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Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

M. Shelbourne, T. Brown, A. H. El-Sagheer and T. Brown, Chem. Commun., 2012, 48, 11184
DOI: 10.1039/C2CC35084J
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Chemical Communications

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Abstract

Supplementary files

Publication details

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

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