Silica-based gene reverse transfection: an upright nanosheet network for promoted DNA delivery to cells

Qingmin Ji; Tomohiko Yamazaki; Nobutaka Hanagata; Michael V. Lee; Jonathan P. Hill; Katsuhiko Ariga

doi:10.1039/C2CC34289H

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Chemical Communications

Issue 68, 2012

Urgent high quality communications from across the chemical sciences.

Impact Factor 6.169 100 Issues per Year Indexed in MEDLINE

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Silica-based gene reverse transfection: an upright nanosheet network for promoted DNA delivery to cells

Qingmin Ji,*^a Tomohiko Yamazaki,^b Nobutaka Hanagata,*^b Michael V. Lee,^a Jonathan P. Hill^a^c and Katsuhiko Ariga*^a^c

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World Premier International (WPI) Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan
E-mail: ARIGA.Katsuhiko@nims.go.jp

Interdisciplinary Laboratory for Nanoscale Science and Technology, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Japan
E-mail: HANAGATA.Nobutaka@nims.go.jp

JST, CREST, 1-1 Namiki, Ibaraki 305-0044, Japan

Chem. Commun., 2012,48, 8496-8498

DOI: 10.1039/C2CC34289H
Received 15 Jun 2012, Accepted 06 Jul 2012
First published online 06 Jul 2012

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Abstract
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A method for substrate-mediated reverse gene transfection was developed using a silica film composed of an upright-sheet network. The silica film with a dense upright-sheet network shows approximately double higher transgene expression efficiency than that of solution-based transfection.

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Qingmin Ji
Tomohiko Yamazaki
Nobutaka Hanagata
Michael V. Lee
Jonathan P. Hill
Katsuhiko Ariga

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Chemical Communications

Silica-based gene reverse transfection: an upright nanosheet network for promoted DNA delivery to cells

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