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Issue 48, 2012
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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

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Abstract

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

Graphical abstract: A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

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Publication details

The article was received on 01 Feb 2012, accepted on 04 Apr 2012, published on 05 Apr 2012 and first published online on 05 Apr 2012


Article type: Communication
DOI: 10.1039/C2CC30710C
Citation: Chem. Commun., 2012,48, 5971-5973
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    A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

    H. Lee, B. C. Kim, M. Oh and J. Kim, Chem. Commun., 2012, 48, 5971
    DOI: 10.1039/C2CC30710C

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