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Issue 42, 2012
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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

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Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.

Graphical abstract: Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

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Publication details

The article was received on 16 Jan 2012, accepted on 03 Apr 2012 and first published on 04 Apr 2012


Article type: Communication
DOI: 10.1039/C2CC30373F
Citation: Chem. Commun., 2012,48, 5127-5129
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    Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

    A. Battisti, M. A. Digman, E. Gratton, B. Storti, F. Beltram and R. Bizzarri, Chem. Commun., 2012, 48, 5127
    DOI: 10.1039/C2CC30373F

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