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Issue 10, 2012
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Surface plasmon resonance imaging of the enzymatic degradation of cellulose microfibrils

Scott G. Allen,a  Oleh M. Tanchak,ab  Amanda Quirk,b  Adam N. Raegen,a  Kyle Reiter,a  Rebecca Whitney,c  Anthony J. Clarke,c  Jacek Lipkowskib  and  John R. Dutcher*a 
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* Corresponding authors

a Department of Physics, University of Guelph, Guelph, Canada N1G 2W1
E-mail: dutcher@uoguelph.ca
Fax: +1 519-836-9967
Tel: +1 519-824-4120 ext. 53950

b Department of Chemistry, University of Guelph, Guelph, Canada N1G 2W1

c Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada N1G 2W1

Abstract

We present the first study of the interaction of a cellulase enzyme mixture with cellulose microfibrils using surface plasmon resonance (SPR) imaging. The cellulose microfibrils, obtained from the bacterium Acetobacter xylinum, were heterogeneously distributed on a thin layer of thioglucose deposited onto a gold film. SPR images collected as a function of time allowed us to observe the adsorption of the enzymes onto both the cellulose microfibrils and the bare surface, and the subsequent degradation of the cellulose microfibrils in real time. In particular, we were able to characterize the decrease in the thickness and variations in thickness of the cellulose microfibril-coated regions with time, and to define a characteristic time for the removal of cellulose from the surface. These results demonstrate the distinct advantage of the SPR imaging technique for measuring the effectiveness of enzymes on cellulose microfibrils and provide useful metrics of enzyme activity that are of relevance to the cellulosic ethanol industry.

Graphical abstract: Surface plasmon resonance imaging of the enzymatic degradation of cellulose microfibrils

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Publication details

The article was received on 16 Jul 2012, accepted on 12 Sep 2012 and first published on 18 Sep 2012


Article type: Paper
DOI: 10.1039/C2AY25762A
Citation: Anal. Methods, 2012,4, 3238-3245
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    Surface plasmon resonance imaging of the enzymatic degradation of cellulose microfibrils

    S. G. Allen, O. M. Tanchak, A. Quirk, A. N. Raegen, K. Reiter, R. Whitney, A. J. Clarke, J. Lipkowski and J. R. Dutcher, Anal. Methods, 2012, 4, 3238
    DOI: 10.1039/C2AY25762A

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