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Issue 12, 2012
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Simultaneous determination of type-A and type-B trichothecenes in rice by UPLC-MS/MS

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Abstract

A reliable and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 5 trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, T-2 toxin, HT-2 toxin) in rice was developed. These target analytes were extracted by acetonitrile/water (84 : 16, v/v), and further purified with solid phase extraction Oasis HLB cartridge. In spiked samples, the mean recoveries ranged from 71.2–102.5% with intra-day and inter-day coefficients of variation less than 11.7 and 12.9%, respectively. Limits of detection (LOD) and quantification (LOQ) were in the range of 0.2–3 μg kg−1 and 0.5–10 μg kg−1, respectively. The results illustrate that this sensitive procedure is applicable for the determination of these trichothecenes in rice.

Graphical abstract: Simultaneous determination of type-A and type-B trichothecenes in rice by UPLC-MS/MS

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Publication details

The article was received on 15 Jul 2012, accepted on 01 Oct 2012 and first published on 02 Oct 2012


Article type: Paper
DOI: 10.1039/C2AY25755F
Citation: Anal. Methods, 2012,4, 4077-4082
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    Simultaneous determination of type-A and type-B trichothecenes in rice by UPLC-MS/MS

    Y. Wang, X. Cao, Y. Li, S. Yang, J. Shen and S. Zhang, Anal. Methods, 2012, 4, 4077
    DOI: 10.1039/C2AY25755F

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