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Issue 6, 2011
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Chromatographic determination of active compounds in topical formulations

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Abstract

The aim of this study was to develop and validate new methods for the determination of active compounds in topical gels. The method for the determination of nonoxinol-9 and trimecaine hydrochloride in lubricant gel by HPLC with UV detection was described. Separation was carried out using a Discovery ZR-PBD analytical column (150 × 4.6 mm, 5 μm), and a mobile phase composed of a mixture of triethylammonium acetate buffer (pH 9; 50 mM) and methanol (22 : 78, v/v). Determination of terpinen-4-ol in lubricant gel was achieved by GC with a flame ionization detector (FID). An Alltech-AT-624 (0.32 mm ID × 30 m, 1.8 μm film thickness) fused-silicacapillary column and helium as the carrier gas, at a flow rate of 28 cm s−1, were employed. The analysis time was less than 7.5 min for HPLC and 5 min for GC analyses. Both methods were successfully applied to the analysis of pharmaceuticals.

Graphical abstract: Chromatographic determination of active compounds in topical formulations

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Publication details

The article was received on 09 Jun 2011, accepted on 18 Aug 2011 and first published on 20 Sep 2011


Article type: Paper
DOI: 10.1039/C1AY05336A
Citation: Anal. Methods, 2011,4, 1525-1529
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    Chromatographic determination of active compounds in topical formulations

    L. Matysová, P. Koblová, L. Galla, H. Sklenářová, L. Havlíková and P. Solich, Anal. Methods, 2011, 4, 1525
    DOI: 10.1039/C1AY05336A

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