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Issue 6, 2012
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Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

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Abstract

Because of its rich π electrons, graphene oxide (GO) can bind with single-stranded DNA (ssDNA) through π-π stacking, but it cannot bind with double-stranded DNA (dsDNA). This different binding ability of GO with DNA has found many applications. By combining this ability with aptamer chemistry, we have developed a highly selective method for the detection of adenosine triphosphate (ATP). The hybrid of ATP aptamer with fluorescein (FAM)-labelled complementary DNA (FAM-DNA) demonstrates a weak affinity for GO and strong fluorescence of FAM-DNA. If ATP is present the strong fluorescence of FAM-DNA is quenched, because the binding of ATP with its aptamer greatly inhibits hybridization of the aptamer with FAM-DNA, and the unhybridized FAM-DNA is adsorbed on to the surface of GO. The quenched fluorescence intensity was found to be in proportion to the concentration of ATP in the range 3–320 μM with a detection limit of 0.45 μM. This method of ATP detection is highly selective and the existence of GTP, CTP and UTP have scarcely effect the determination.

Graphical abstract: Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

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Publication details

The article was received on 16 Feb 2012, accepted on 27 Mar 2012 and first published on 28 Mar 2012


Article type: Paper
DOI: 10.1039/C2AY25166C
Citation: Anal. Methods, 2012,4, 1662-1666
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    Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

    W. D. Pu, L. Zhang and C. Z. Huang, Anal. Methods, 2012, 4, 1662
    DOI: 10.1039/C2AY25166C

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