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Issue 6, 2012
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Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

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Abstract

Encapsulation of materials into capsules becomes discrete at small scales. We propose a measurement and analysis method of discrete encapsulation of nanomaterials in colloidal capsules, with the volume of fL to pL, using the fluorescence flow cytometry. Encapsulation of nano-sized polymer beads and lambda-phage DNA into lipid vesicles (liposomes) is evaluated as a model system. By using fluorescence markers, vesicle volume, membrane quantity and nanomaterials in a large number of individual vesicles are measured simultaneously. We show that the number of encapsulated material (k = 0, 1, 2, …) can be evaluated from the quantized fluorescence intensity distribution, which is then compared with the Poisson statistics. As a result, probability of encapsulating beads and λDNA exhibits characteristic trends that depend on the vesicle volume, lamellarity, and electrostatic interaction, which should reflect the physical interaction in the vesicle formation processes. This measurement and analysis will be a powerful tool in designing the colloidal capsules used in many scientific and industrial applications.

Graphical abstract: Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

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Publication details

The article was received on 31 Jan 2012, accepted on 06 Apr 2012 and first published on 10 Apr 2012


Article type: Paper
DOI: 10.1039/C2AY25105A
Citation: Anal. Methods, 2012,4, 1648-1655
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    Statistical analysis of discrete encapsulation of nanomaterials in colloidal capsules

    T. Sakakura, K. Nishimura, H. Suzuki and T. Yomo, Anal. Methods, 2012, 4, 1648
    DOI: 10.1039/C2AY25105A

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