Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2012
Previous Article Next Article

Determination of endogenous salsolinol and N-methylsalsolinol in rat brain by LC-ESI-QQQ

Author affiliations

Abstract

Salsolinol (Sal) and N-methylsalsolinol (NMSal) are endogenous neurotoxins in the brain, which are the key causative agents of the death of dopaminergic neurons. Due to their strong polarity, they have weak retention and are difficult to quantify using the C18 column. In the present study, a sensitive and selective LC/MS method for the determination of Sal and NMSal was developed and validated using a novel pentafluorophenylpropyl column performed on high performance liquid chromatography electrospray ionisation triple quadrupole. The calibration curve was linear over the range of 0.098–25 nM and 3.91–250 nM for Sal and NMSal, respectively. The lower limit of quantitation is 0.098 nM and 3.91 nM for Sal and NMSal. The inter-day and intra-day precision and accuracy for all samples were acceptable. Furthermore, the assay has also been applied for the determination of Sal and NMSal in rat brain.

Graphical abstract: Determination of endogenous salsolinol and N-methylsalsolinol in rat brain by LC-ESI-QQQ

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 02 Sep 2011, accepted on 17 Apr 2012 and first published on 18 Apr 2012


Article type: Paper
DOI: 10.1039/C2AY05551A
Citation: Anal. Methods, 2012,4, 2027-2030
  •   Request permissions

    Determination of endogenous salsolinol and N-methylsalsolinol in rat brain by LC-ESI-QQQ

    Y. Zhang, Y. Li, Z. Zhang, C. Lai and Y. Deng, Anal. Methods, 2012, 4, 2027
    DOI: 10.1039/C2AY05551A

Search articles by author