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Issue 20, 2012
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A study of enzymatic activity in cell cultures via the analysis of volatile biomarkers

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Abstract

Aldehyde dehydrogenase (ALDH) enzymes are responsible for the metabolism of aldehydes, including acetaldehyde (AA), and are linked to disease. We describe a method to study ALDH activity in cell cultures involving the measurement of AA concentrations in the gas/vapour phase. This has been achieved using selected ion flow tube mass spectrometry (SIFT-MS), developed for the rapid quantification of trace gases in humid media. Human cells of the hepG2 hepatocellular carcinoma cell line and primary bone marrow-derived mesenchymal stem cells (hMSCs) depleted AA from the culture media, but the application of ALDH inhibitors diethylaminobenzaldehyde (DEAB) and disulfiram (DSF), suppressed this depletion or in some cases resulted in elevated AA concentrations. Further, the cells were shown to reduce the dimethyl sulphoxide (DMSO) to dimethyl sulphide, which is mediated by methionine sulfoxide reductase A (MsrA) enzymes. Interestingly, this process was also inhibited by DEAB and DSF. The results of this study indicate that SIFT-MS gas phase analysis could be applied to the study of volatile metabolites of intracellular enzyme reactions, this having potential utility in disease research and drug discovery.

Graphical abstract: A study of enzymatic activity in cell cultures via the analysis of volatile biomarkers

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Publication details

The article was received on 18 Jun 2012, accepted on 21 Aug 2012 and first published on 29 Aug 2012


Article type: Paper
DOI: 10.1039/C2AN35815H
Citation: Analyst, 2012,137, 4677-4685
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    A study of enzymatic activity in cell cultures via the analysis of volatile biomarkers

    T. W. E. Chippendale, B. Hu, A. J. El Haj and D. Smith, Analyst, 2012, 137, 4677
    DOI: 10.1039/C2AN35815H

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