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Issue 22, 2012
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Real time measurement of myocardial oxygen dynamics during cardiac ischemia–reperfusion of rats

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Abstract

Because oxygen plays a critical role in the pathophysiology of myocardial injury during subsequent reperfusion, as well as ischemia, the accurate measurement of myocardial oxygen tension is crucial for the assessment of myocardial viability by ischemia–reperfusion (IR) injury. Therefore, we utilized a sol–gel derived electrochemical oxygen microsensor to monitor changes in oxygen tension during myocardial ischemia–reperfusion. We also analyzed differences in oxygen tension recovery in post-ischemic myocardium depending on ischemic time to investigate the correlation between recovery parameters for oxygen tension and the severity of IR injury. An oxygen sensor was built using a xerogel-modified platinum microsensor and a coiled Ag/AgCl reference electrode. Rat hearts were randomly divided into 5 groups: control (0 min ischemia), I-10 (10 min ischemia), I-20 (20 min ischemia), I-30 (30 min ischemia), and I-40 (40 min ischemia) groups (n = 3 per group, respectively). After the induction of ischemia, reperfusion was performed for 60 min. As soon as the ischemia was initiated, oxygen tension rapidly declined to near zero levels. When reperfusion was initiated, the changes in oxygen tension depended on ischemic time. The normalized peak level of oxygen tension during the reperfusion episode was 188 ± 27 in group I-10, 120 ± 24 in group I-20, 12.5 ± 10.6 in group I-30, and 1.24 ± 1.09 in group I-40 (p < 0.001, n = 3, respectively). After 60 min of reperfusion, the normalized restoration level was 129 ± 30 in group I-10, 88 ± 4 in group I-20, 3.40 ± 4.82 in group I-30, and 0.99 ± 0.94 in group I-40 (p < 0.001, n = 3, respectively). The maximum and restoration values of oxygen tension in groups I-30 and I-40 after reperfusion were lower than pre-ischemic values. In particular, oxygen tension in the I-40 group was not recovered at all. These results were also demonstrated by TTC staining. We suggest that these recovery parameters could be utilized as an index of tissue injury and severity of ischemia. Therefore, quantitative measurements of oxygen tension dynamics in the myocardium would be helpful for evaluation of the cardioprotective effects of therapeutic treatments such as drug administration.

Graphical abstract: Real time measurement of myocardial oxygen dynamics during cardiac ischemia–reperfusion of rats

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Publication details

The article was received on 14 Feb 2012, accepted on 08 Sep 2012 and first published on 10 Sep 2012


Article type: Paper
DOI: 10.1039/C2AN35208G
Citation: Analyst, 2012,137, 5312-5319
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    Real time measurement of myocardial oxygen dynamics during cardiac ischemia–reperfusion of rats

    G. Lee, S. K. Kim, S. W. Kang, O. Kim, S. Chae, S. Choi, J. H. Shin, H. Park and J. Chung, Analyst, 2012, 137, 5312
    DOI: 10.1039/C2AN35208G

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