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Issue 9, 2012
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A simple colorimetric detection of DNA methylation

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Abstract

In this work, we describe a simple colorimetric method to detect DNA methylation. Adenomatous polyposis coli (APC) with a small CpG region containing methylated cytosine (methylated APC) was synthesized and tested. Methylated APC was first captured and enriched by anti-5-methylcytosine monoclonal antibody conjugated magnetic microspheres (MMPs). Then a probe partly complementary to the APC sequence was added, resulting in the formation of DNA duplexes. The microsphere-captured probe was then released by heat denaturation and added into unmodified gold nanoparticle (AuNP) solution. Colorimetric detection was performed by salt-induced aggregation. The limit of detection is 80 fmol. Semi-quantitative analysis was done with a UV/Vis spectrophotometer by recording the absorbance of AuNP solution at 520 nm. Thus, this method provides a simple, rapid and quantitative tool for DNA methylation detection.

Graphical abstract: A simple colorimetric detection of DNA methylation

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Publication details

The article was received on 10 Jan 2012, accepted on 05 Mar 2012 and first published on 05 Mar 2012


Article type: Communication
DOI: 10.1039/C2AN35043B
Citation: Analyst, 2012,137, 2032-2035
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    A simple colorimetric detection of DNA methylation

    C. Ge, Z. Fang, J. Chen, J. Liu, X. Lu and L. Zeng, Analyst, 2012, 137, 2032
    DOI: 10.1039/C2AN35043B

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