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Issue 12, 2012
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Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation

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Abstract

The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent re-crystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates.

Graphical abstract: Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation

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Publication details

The article was received on 28 Dec 2011, accepted on 24 Apr 2012 and first published on 25 Apr 2012


Article type: Paper
DOI: 10.1039/C2AN16310A
Citation: Analyst, 2012,137, 2866-2870
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    Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation

    R. Prucek, V. Ranc, L. Kvítek, A. Panáček, R. Zbořil and M. Kolář, Analyst, 2012, 137, 2866
    DOI: 10.1039/C2AN16310A

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