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Issue 9, 2012
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Oligonucleotide analysis by nanoparticle-assisted laser desorption/ionization mass spectrometry

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Abstract

We analyzed oligonucleotides by nanoparticle-assisted laser desorption/ionization (nano-PALDI) mass spectrometry (MS). To this end, we prepared several kinds of nanoparticles (Cr-, Fe-, Mn-, Co-based) and optimized the nano-PALDI MS method to analyze the oligonucleotides. Iron oxide nanoparticles with diammonium hydrogen citrate were found to serve as an effective ionization-assisting reagent in MS. The mass spectra showed both [M − H] and [M + xMe2+ – H] (Me: transition metal) peaks. The number of metal-adducted ion signals depended on the length of the oligonucleotide. This phenomenon was only observed using bivalent metal core nanoparticles, not with any other valency metal core nanoparticles. Our pilot study demonstrated that iron oxide nanoparticles could easily ionize samples such as chemical drugs and peptides as well as oligonucleotides without the aid of an oligonucleotide-specific chemical matrix (e.g., 3-hydroxypicolinic acid) used in conventional MS methods. These results suggested that iron-based nanoparticles may serve as the assisting material of ionization for genes and other biomolecules.

Graphical abstract: Oligonucleotide analysis by nanoparticle-assisted laser desorption/ionization mass spectrometry

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Publication details

The article was received on 09 Dec 2011, accepted on 28 Jan 2012 and first published on 01 Feb 2012


Article type: Communication
DOI: 10.1039/C2AN16237G
Citation: Analyst, 2012,137, 2006-2010
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    Oligonucleotide analysis by nanoparticle-assisted laser desorption/ionization mass spectrometry

    S. Taira, I. Osaka, S. Shimma, D. Kaneko, T. Hiroki, Y. Kawamura-Konishi and Y. Ichiyanagi, Analyst, 2012, 137, 2006
    DOI: 10.1039/C2AN16237G

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