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Issue 14, 2012
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Rapid microRNA detection using power-free microfluidic chip: coaxial stacking effect enhances the sandwich hybridization

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Abstract

We present a new method for rapid microRNA detection with a small volume of sample using the power-free microfluidic device driven by degassed PDMS. Target microRNA was detected by sandwich hybridization taking advantage of the coaxial stacking effect. This method allows us to detect miR-21 in 20 min with a 0.5 μL sample volume at a limit of detection of 0.62 nM. Since microRNAs can act as cancer markers, this method might substantially contribute to future point-of-care cancer diagnosis.

Graphical abstract: Rapid microRNA detection using power-free microfluidic chip: coaxial stacking effect enhances the sandwich hybridization

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Publication details

The article was received on 24 Nov 2011, accepted on 06 May 2012 and first published on 22 May 2012


Article type: Communication
DOI: 10.1039/C2AN16154K
Citation: Analyst, 2012,137, 3234-3237
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    Rapid microRNA detection using power-free microfluidic chip: coaxial stacking effect enhances the sandwich hybridization

    H. Arata, H. Komatsu, A. Han, K. Hosokawa and M. Maeda, Analyst, 2012, 137, 3234
    DOI: 10.1039/C2AN16154K

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