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Issue 8, 2012
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Coherent two-dimensional infrared spectroscopy: Quantitative analysis of protein secondary structure in solution

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Abstract

We present a method to quantitatively determine the secondary structure composition of globular proteins using coherent two-dimensional infrared (2DIR) spectroscopy of backbone amide I vibrations (1550–1720 cm−1). Sixteen proteins with known crystal structures were used to construct a library of 2DIR spectra, and the fraction of residues in α-helix, β-sheet, and unassigned conformations was determined by singular value decomposition (SVD) of the measured two-dimensional spectra. The method was benchmarked by removing each individual protein from the set and comparing the composition extracted from 2DIR against the composition determined from the crystal structures. To highlight the increased structural content extracted from 2DIR spectra a similar analysis was also carried out using conventional infrared absorption of the proteins in the library.

Graphical abstract: Coherent two-dimensional infrared spectroscopy: Quantitative analysis of protein secondary structure in solution

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Publication details

The article was received on 29 Oct 2011, accepted on 10 Feb 2012 and first published on 08 Mar 2012


Article type: Paper
DOI: 10.1039/C2AN16031E
Citation: Analyst, 2012,137, 1793-1799
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    Coherent two-dimensional infrared spectroscopy: Quantitative analysis of protein secondary structure in solution

    C. R. Baiz, C. S. Peng, M. E. Reppert, K. C. Jones and A. Tokmakoff, Analyst, 2012, 137, 1793
    DOI: 10.1039/C2AN16031E

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