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Issue 6, 2012
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Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

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Abstract

We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.

Graphical abstract: Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

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Publication details

The article was received on 06 Oct 2011, accepted on 30 Dec 2011 and first published on 19 Jan 2012


Article type: Paper
DOI: 10.1039/C2AN15930A
Citation: Analyst, 2012,137, 1351-1362
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    Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

    G. M. Credo, X. Su, K. Wu, O. H. Elibol, D. J. Liu, B. Reddy, T. Tsai, B. R. Dorvel, J. S. Daniels, R. Bashir and M. Varma, Analyst, 2012, 137, 1351
    DOI: 10.1039/C2AN15930A

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