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Issue 6, 2012
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Separation of bacteria with imprinted polymeric films

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Abstract

Separation of compounds out of complex mixtures is a key issue that has been solved for small molecules by chromatography. However, general methods for the separation of large bio-particles, such as cells, are still challenging. We demonstrate integration of imprinted polymeric films (IPF) into a microfluidic chip, which preferentially capture cells matching an imprint template, and separate strains of cyanobacteria with 80–90% efficiency, despite a minimal difference in morphology and fluorescence, demonstrating its general nature. It is currently thought that the imprinting process, conducted while the polymer cures, transfers chemical information of the cell's external structure to the substrate. Capture specificity and separation can be further enhanced by orienting the imprints parallel to the flow vector and tuning the pH to a lower range.

Graphical abstract: Separation of bacteria with imprinted polymeric films

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Publication details

The article was received on 05 Oct 2011, accepted on 25 Jan 2012 and first published on 10 Feb 2012


Article type: Paper
DOI: 10.1039/C2AN15927A
Citation: Analyst, 2012,137, 1495-1499
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    Separation of bacteria with imprinted polymeric films

    R. Schirhagl, E. W. Hall, I. Fuereder and R. N. Zare, Analyst, 2012, 137, 1495
    DOI: 10.1039/C2AN15927A

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