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Issue 4, 2013
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Detection of cancer cells using a peptide nanotubefolic acid modified graphene electrode

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Abstract

This article describes the preparation of a graphene electrode modified with a new conjugate of peptide nanotubes and folic acid for the selective detection of human cervical cancer cells over-expressing folate receptors. The functionalization of peptide nanotubes with folic acid was confirmed by fluorescence microscopy and atomic force microscopy. The peptide nanotubefolic acid modified graphene electrode was characterized by scanning electron microscopy and cyclic voltammetry. The modification of the graphene electrode with peptide nanotubefolic acid led to an increase in the current signal. The human cervical cancer cells were bound to the modified electrode through the folic acid–folate receptor interaction. Cyclic voltammograms in the presence of [Fe(CN)6]3−/4− as a redox species demonstrated that the binding of the folate receptor from human cervical cancer cells to the peptide nanotubefolic acid modified electrode lowered the electron transfer resulting in a decrease in the measured current. A detection limit of 250 human cervical cancer cells per mL was obtained. Control experiments confirmed that the peptide nanotubefolic acid electrode specifically recognized folate receptors. The modified electrode described here opens up new possibilities for future applications in early stage diagnoses of diseases where cells over-express folate receptors, such as in cancer or leishmaniasis disease.

Graphical abstract: Detection of cancer cells using a peptide nanotube–folic acid modified graphene electrode

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Publication details

The article was received on 14 Aug 2012, accepted on 26 Oct 2012 and first published on 26 Oct 2012


Article type: Paper
DOI: 10.1039/C2AN36121C
Citation: Analyst, 2013,138, 1026-1031
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    Detection of cancer cells using a peptide nanotubefolic acid modified graphene electrode

    J. J. Castillo, W. E. Svendsen, N. Rozlosnik, P. Escobar, F. Martínez and J. Castillo-León, Analyst, 2013, 138, 1026
    DOI: 10.1039/C2AN36121C

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